FIG. 7.

Sulfonylurea receptor 1 (SUR1) expression in rat contusion- traumatic brain injury (TBI). (A, B) Coronal image of contusion (A) and coronal section immunolabeled for glial fibrillary acidic protein (GFAP; red) and stained for TUNEL (green; B), showing that the hemorrhagic core is largely devoid of GFAP immunoreactivity, but shows widespread TUNEL labeling; the white line in (A) depicts the output of the algorithm-based segmentation protocol used to identify the hemorrhagic lesion area (see Methods). (C, D) Quantification of total SUR1 expression in core (red) vs. penumbra (blue) as a function of time post-contusion-TBI; five rats per time-point; the labels “microvessels,” “astrocytes,” and “macrophages” in (C) are based on high magnification views (D), showing that: 1) early in the core, SUR1 is most prominent in elongated structures consistent with microvessels; 2) later in the core, SUR1 is most prominent in small round cells; and 3) at all times in the penumbra, SUR1 is most prominent in stellate cells consistent with astrocytes.