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. 2019 Apr;189(4):868–885. doi: 10.1016/j.ajpath.2018.12.010

Figure 9.

Figure 9

Analysis of cell organization and collagen fiber orientation in JNK1/2 dKO and control (CTRL) mice. A–L: Hematoxylin and eosin staining of embryonic day (E) 15.5 (A–F) and E17.5 (G–L) intervertebral disk showing larger, unorganized cells in the inner annulus fibrosus of dKO mice (C, F, I, and L) (red arrows; F and L) compared with CTRL (A, D, G, and J) and heterozygous (Het) littermates (B, E, H, and K) (black arrows; D, E, J, and K). Boxed areas in A–C are shown in higher-magnification in D–F, respectively, and boxed areas in G–I are shown in higher-magnification in J–L, respectively. M–O: Safranin O/fast green stain of E17.5 vertebral column (M–O) showing fusion of posterior spinal elements (black circles) in the dKO (O). The images represent sagittal sections through the vertebral column. n = 5 E15.5 dKO (C and F); n = 6 E15.5 CTRL/Het (A, B, D, and E); n = 4 E17.5 dKO (I, L, O); n = 8 E17.5 CTRL/Het (G, H, J, K, M, and N). Scale bars: 20 μm (A–C, G–I, and M–O); 10 μm (D–F and J–L). SC, spinal cord.