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. 2019 Apr;189(4):797–812. doi: 10.1016/j.ajpath.2018.12.016

Figure 1.

Figure 1

Effect of pharmacologic inhibition of NF-κB on lipopolysaccharide (LPS)-induced increase in intestinal epithelial tight junction permeability. A: Filter-grown Caco-2 monolayers were treated with 300 pg/mL LPS for a 5-day experimental period. Pharmacologic inhibition of NF-κB by 10 μm ammonium pyrrolidinedithiocarbamate (PDTC) inhibited the LPS (physiological dose of 300 pg/mL)-induced drop in Caco-2 transepithelial electrical resistance. B: Pharmacologic inhibition of NF-κB by PDTC prevented the LPS-induced increase in inulin flux. NF-κB inhibitor PDTC was added 1 hour before LPS treatment. All experimental treatments were renewed every 24 hours for the 5-day experimental period. Data are expressed as means ± SEM. n = 4 independent experiments. ∗∗∗P < 0.001 versus control; †††P < 0.001 versus LPS.