Table 3.
Concentration of diazinon (DZ), hydroxypyrimidine (PYR), and hydroxypyrimidine-glucuronide (PYR-gluc) in media and slices exposed to 340 nmoles DZ/well (200 μM). Media only samples were not incubated with a slice. Media and Slice Phase I samples were taken from the same well. Slice Phase II samples were taken from the separate well. Data is from three separate tests. Values represent mean of three replicate samples.
| Time hours |
Media only (nmoles/well) DZ |
Media (nmoles/well) | Slice (nmoles/slice) | Total nmoles Media+Slice |
Total as % Media only |
||||
|---|---|---|---|---|---|---|---|---|---|
| DZ | Phase I PYR |
Phase II PYR-gluc |
DZ | Phase I PYR |
Phase II PYR-gluc |
||||
| TEST 1 | |||||||||
| 0 | 312.2 | 312.2 | 0 | 0 | 0 | 0 | 0 | 312.2 | 100 |
| 1 | 311.9 | 138.5 | 2.7 | 5.2 | 22.9 | * | 0.5 | 169.8 | 54 |
| 3 | 312.9 | 136.7 | 4.8 | 5.5 | 35.5 | * | 0.8 | 183.3 | 59 |
| 6 | 303.0 | 131.9 | 10.1 | 3.8 | 44.9 | * | 0.8 | 191.5 | 63 |
| 24 | 307.2 | 119.4 | 20.9 | 6.3 | 40.1 | * | 0.3 | 187.0 | 61 |
| TEST 2 | |||||||||
| 0 | 316.9 | 316.9 | 0 | 0 | 0 | 0 | 0 | 316.9 | 100 |
| 3 | 283.7 | 165.5 | 3.4 | 1.5 | 57.2 | * | 1.5 | 229.1 | 81 |
| 24 | 313.7 | 150.0 | 20.2 | 7.4 | 69.4 | * | 1.8 | 248.8 | 79 |
| TEST 3 | |||||||||
| 0 | 339.2 | 339.2 | 0 | NM | 0 | 0 | NM | 339.2 | 100 |
| 3 | 343.2 | 195.3 | 7.9 | NM | 42.6 | * | NM | 245.8 | 72** |
| 6 | 336.3 | 191.3 | 13.0 | NM | 36.0 | * | NM | 240.3 | 71** |
| 24 | 334.7 | 176.2 | 22.6 | NM | 38.8 | * | NM | 237.6 | 71** |
*
peak was detected but not quantifiable (S/N<2)
NM=Not Measured
**
value does not include contribution from PYR-gluc