Table 2.
Strains
| Strain | Genotype and Description | Reference* |
|---|---|---|
| E. coli | ||
| OmniMAX – 2 T1R | F´ [proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD. | Invitrogen |
| XL1-Blue | endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 [F’ proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+) Tn10(TetR)] | |
| HB101/pRK24 | F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | (Trieu-Cuot et al., 1987) |
| EC3272 | XL1-Blue / pDSW1728 (Ptet::rfp cat) | (Ransom et al., 2015) |
| RAN473 | OmniMAX / pRAN473 (Ptet::rfp –MCS cat) | (Ransom et al., 2015) |
| C. difficile | ||
| JIR8094 | Spontaneous erythromycin-sensitive derivative of strain 630 (Ribotype 012) | (O’Connor et al., 2006) |
| CD630Δerm | Spontaneous erythromycin-sensitive derivative of strain 630 (Ribotype 012) | (Hussain et al., 2005) |
| CD196 | Wild-type C. difficile strain from France (ribotype 027) | |
| NAP07 | Wild-type C. difficile strain ribotype 078 | |
| NAP08 | Wild-type C. difficile strain ribotype 078 | |
| R20291 | Wild-type C. difficile strain from UK outbreak (ribotype 027) | |
| RAN820 | NAP08 / pRAN737 (PtcdA::rfp cat) | |
| RAN828 | JIR8094 / pRAN737 (PtcdA::rfp cat) | |
| RAN829 | JIR8094 / pRAN737 (PpdaV::rfp cat) | (Ransom et al., 2015) |
| RAN912 | JIR8094 / pRAN841 (PtcdB::rfp cat) | |
| RAN913 | JIR8094 / pRAN842 (PtcdR::rfp cat) | |
| RAN925 | R20291 / pRAN737 (PtcdA::rfp cat) | |
| RAN934 | CD196 / pRAN737 (PtcdA::rfp cat) | |
| RAN1101 | NAP07 / pRAN737 (PtcdA::rfp cat) | |
| GMK134 | CD630Δerm / pRAN737 (PtcdA::rfp cat) | |
| RAN1116 | JIR8094 codY330::ltrB::ermB | |
| RAN1123 | JIR8094 tcdR142::ltrB::ermB | |
| RAN1121 | JIR8094 sigH123::ltrB::ermB | |
| RAN1120 | JIR8094 ccpA133::ltrB::ermB | |
| CDE1774 | JIR8094 agrB188::ltrB::ermB | |
| RAN1124 | JIR8094 codY330::ltrB::ermB / pRAN737 (PtcdA::rfp cat) | |
| RAN1127 | JIR8094 tcdR142::ltrB::ermB / pRAN737 (PtcdA::rfp cat) | |
| RAN1126 | JIR8094 sigH123::ltrB::ermB / pRAN737 | |
| RAN1125 | JIR8094 ccpA133::ltrB::ermB / pRAN737 | |
| CDE2770 | JIR8094 agrB188::ltrB::ermB / pRAN737 | |
| TCD20 | JIR8094 csfV63::ltrB::ermB | (Ho et al., 2014) |
| RAN1129 | JIR8094 tcdR142::ltrB::ermB / pRAN1032 | |
| RT1566 | R20291 sigD228::erm | (Anjuwon-Foster and Tamayo, 2017) |
| GMK129 | R20291 sigD228::erm / pRAN737 | |
| GMK130 | R20291 sigD228::erm / pGK110 |
*
This study unless otherwise noted.