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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 14;110(4):533–549. doi: 10.1111/mmi.14107

Table 3.

Plasmids

Plasmid Relevant Features Reference*
pRPF185 E. coli-C. difficile shuttle vector with tetracycline-inducible promoter. Ptet::gusA cat CD6ori RP4oriT-traJ pMB1ori (Fagan and Fairweather, 2011)
pBL100 E. coli-C. difficile shuttle vector for creating C. difficile mutants using TargeTron mutagenesis. ltrB::ermB::RAM ltrA cat bla CD6ori RP4oriT pMBlori (Bouillaut et al., 2013)
pDSW1728 Ptet::rfp cat (Ransom et al., 2015)
pCE536 pBL100 targeted to codY330
pRAN737 pDSW1728 derivative with PtcdA::rfp
pRAN841 pDSW1728 derivative with PtcdB::rfp
pRAN842 pDSW1728 derivative with PtcdR::rfp
pRAN1018 PtcdA::rfp / PpdaV::tcdR
pRAN1032 PtcdA::rfp / Ptet::tcdR
pTHE583 pBL100 targeted to agrB188
pCE540 pBL100 targeted to sigH123
pCE541 pBL100 targeted to ccpA133
pRAN1034 pBL100 targeted to tcdR142
pGK110 PtcdA::rfp / Ptet::sigD
*

This study unless otherwise noted.