Fig. 3.

Concentrations of Th17-related cytokines in tested groups. The figure depicts the Log₁₀ concentration values of estimated Th17-related cytokines in serum in the CTRL, SA, and SA + SD groups, respectively. Th17-related cytokines were quantified by flow cytometry (see methods). The concentration values of proinflammatory Th17-related cytokines are expressed as the mean ± SEM, as described: CTRL; IL-17A (25.9 ± 2.1 pg/mL); IL-17F (22.7 ± 1.8 pg/mL); IL-21, ND; IL-22, ND. SA; IL-17A (84.6 ± 1.3 pg/mL), IL-17F (38.1 ± 3.7 pg/mL), IL-21 (87.9 ± 2.4 pg/mL), IL-22 (88.9 ± 2.7 pg/mL). SA + SD; IL-17A (109. 9 ± 1 8.3 pg/mL), IL-17F (59.1 ± 5.5 pg/mL), IL-21 (92.6 ± 4.5 pg/mL), IL-22 (113.5 ± 8.6 pg/mL). The non-parametric, t-test analysis with Welch’s correction was used to estimate the p values for each Th2-related cytokine assayed among the studied groups. (★) non-determined values; (*) p ≤ 0.05; (**); p ≤ 0.001; (***) p ≤ 0.0001. Significant differences were established at a p < 0.05. Abbreviations: ND, non-determined; SA + SD, severe anxiety plus comorbid severe depression; SA, severe anxiety; CTRL; control. Data were calculated using GraphPad v.7