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. 2019 Apr 3;21:87. doi: 10.1186/s13075-019-1865-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The effects of HKI/II on the glycolytic activities and cell survival of RASFs. a The silencing efficiency was confirmed by Western blot in RASFs (n = 3). After transfection with siHKI, siHKII or siHKI/II (50 nM) or its control (siCtrl) for 24 h, glucose uptake (b) and HKs activity (c) were determined in RASFs (n = 3). d Glucose concentrations in the incubation media of RASFs (n = 6) were periodically monitored. e l-lactate level was measured in the supernatant of RASFs (n = 6) after indicated treatments. The viability (f) and apoptosis (g) of RASFs (n = 3) were analyzed by MTS, Annexin X staining, and trypan blue. Data represent the mean ± SE. The statistical significance of differences between siCtrl and siHKI/II group was determined. *p < 0.05, **p < 0.01 significantly different; ns insignificant difference. Asterisk in d indicates siHKI/II vs siCtrl, number sign indicates siHKI vs siCtrl, and star indicates siHK II vs siCtrl (*p < 0.05, ^#p < 0.05, and ^☆p < 0.05)