Fig. 4.

HKI/II regulate cytokine and chemokine production of RASFs and THP1. After transfection with siHKI, siHKII or siHKI/II or siCtrl (50 nM) for 6 h, RASFs (n = 3) were treated with IL-1β and TNF-α for 24 h. Secretion of IL-6 (a) and IL-8 (b) in culture supernatant was detected by ELISA. CXCL9 (c), CXCL10 (d), and CXCL11 (e) mRNA were assayed by RT-qPCR. f HK-I and II mRNA in THP1 cells were evaluated by RT-qPCR. g The production of TNF-α and IL-1β were also determined by ELISA in siHKI, siHKII or siHKI/II-transfected THP1 with the stimulation of LPS. Results in THP-1 are technical replicates. Data represent the mean ± SE. *p < 0.05, **p < 0.01 significantly different, ns insignificant difference