Fig. 1.

CircCENND2A is a hypoxia-responsive circRNA in glioma cells. a Hypoxia-associated circRNAs in glioma were screened by comparing the differentially expressed circRNA profiles between glioma and normal tissues (GSE86202) and by comparing the differentially expressed mRNA profiles in U87MG cells between hypoxia and normoxia (GSE45301). b Fold changes of the screened circRNAs under hypoxia versus normoxia were measured by qPCR in U87MG cells. c The levels of DENND2A mRNA or circRNA in normoxic or hypoxic U87MG cells treated with or without RNase R were measured by qPCR. d RNA fluorescence in situ hybridization showed the abundance and localization of circDENND2A in normoxic or hypoxic U87MG cells. DAPI solution was used to stain the nuclei. e The levels of DENND2A circRNA in normoxic or hypoxic SVGp12, U87MG, and A172 cells were measured by qPCR. Data are shown as mean ± SD (three independent repeats). * p < 0.05, ** p < 0.01, *** p < 0.001