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. 2019 Apr 2;16:69. doi: 10.1186/s12974-019-1455-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — TGFβ1-induced CCL2 and CX3CL1 modulation in neurons promote microglia activation. a Relative IL-1β and (b) TNFα mRNA expression in EOC-20 cells treated with C021, INCB, or recombinant CX3CL1 following supplementation with conditioned media from HT-22 cells treated with recombinant TGFβ1. c Phagocytosis activity, reported as fluorescence intensity of phagocytized E. coli bioparticles, in EOC-20 cells treated with C021, INCB, or recombinant CX3CL1 following supplementation with conditioned media from HT-22 cells treated with recombinant TGFβ1. *p < 0.05 compared to EOC-20 supplemented with basal HT-22 conditioned media. ^#p < 0.05 compared to EOC-20 supplemented with TGFβ1-treated HT-22 conditioned media. n = 3 for mRNA analyses and n = 7 for phagocytosis activity assay