Table 1.
Antinociceptive effects of PECN assessed using the hot plate test in mice
| Group | Dose (mg/kg) | Latency of discomfort(s) at respective time interval (min) | ||||||
|---|---|---|---|---|---|---|---|---|
| 0 min | 60 min | 90 min | 120 min | 150 min | 180 min | 210 min | ||
| 10% DMSO | 6.29 ± 0.15 | 6.88 ± 0.29 | 6.89 ± 0.31 | 6.28 ± 0.12 | 6.76 ± 0.43 | 6.67 ± 0.33 | 6.46 ± 0.12 | |
| PECN | 100 | 6.41 ± 0.40 | 8.95 ± 0.87*a | 8.23 ± 0.35*a | 8.38 ± 0.48*a | 8.11 ± 0.66*a | 8.41 ± 0.58*a | 7.57 ± 0.63 |
| 250 | 5.70 ± 0.16 | 9.71 ± 0.84#b | 8.29 ± 0.40#b | 8.92 ± 0.50#ba | 8.17 ± 0.33*b | 7.77 ± 0.31*a | 7.89 ± 0.28*a | |
| 500 | 6.37 ± 0.18 | 10.28 ± 0.72§c | 9.52 ± 0.47#b | 8.96 ± 0.23#a | 9.47 ± 0.31#b | 9.55 ± 0.40#b | 9.06 ± 0.51#b | |
| MOR | 5 | 6.02 ± 0.15 | 17.00 ± 0.90§c | 18.42 ± 0.47§c | 17.25 ± 0.93§c | 13.47 ± 1.31§c | 11.87 ± 1.04§c | 11.15 ± 0.71§c |
Data expressed are the mean ± SEM of reaction time (sec) of six mice
Statistical analysis was performed using two–way ANOVA followed by the Bonferroni post hoc test
*p < 0.05, #p < 0.01, §p < 0.001, compared to control group within the respective column
ap < 0.05, bp < 0.01, cp < 0.001, compared to control group within the respective row
DMSO Dimetyl sulfoxide, PECN Petroleum ether extract of C. nutans, MOR morphine
Mice were treated (p.o) with vehicle (10 mL/kg), PECN (100, 250, 500 mg/kg), or MOR (5 mg/kg) for 60 mins prior to subjection to the test