Fig. 2.

Males have increased tissue macrophage responses in high-fat diet that persist in weight loss. WT male and female C57Bl/6J mice were placed on high-fat diet (HFD) starting at 6 weeks of age. After 12 weeks of diet, a group of these mice was switched to normal chow to mimic dietary weight loss (WL). A control group remained on normal chow the entire time (ND). Stromal vascular fractions were isolated and adipose tissue macrophages (ATM) and dendritic cells (DCs) assessed by flow cytometry in a GWAT, b IWAT, and c tissue macrophages and DCs in the liver. d Bone marrow granulocyte-macrophage progenitors (GMPs) were also evaluated by flow cytometry. Statistics from diet and sex interaction in box. *p < 0.05, ***p < 0.005, ****p < 0.001. #p < 0.05, ### p < 0.005, and ####p < 0.001 for comparisons between sexes of the same diet group. N = 4–8 per group