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. 2019 Apr 3;10:13. doi: 10.1186/s13100-019-0155-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overall scheme of the expression cassettes tested in this study. The tested sequences of Tc1-like, mariner-like and hobo elements (a, b and c) are divided into a blue region containing the transposase binding sites (depicted as dashed boxes) and sequences situated between the upstream TIRs and the transposase ORFs in these transposons (green region). These fragments are directly fused to a luciferase reporter gene (yellow). The LTR-retrotransposon sequences (d) are depicted with their canonical U3-R-U5 structure (more details are given in the main text). The organization of the negative (e) and positive (f) construct are also reported. Drawings are not in scale