Fig. 6.

ENS-653 downregulation induces a G1 cell cycle arrest with no impact on apoptosis. a Flow cytometry was performed in IHH4 cells transfected with Si-653 (siRNA silencing ENS-653) or Si-NC (negative control). The bar chart represents the percentage of IHH4 cells in G0/G1, S or G2/M phase. b Flow cytometry was used to detect the apoptotic cells in IHH4 cells transfected with Si-653 or Si-NC. LR, early apoptotic cells; UR, terminal apoptotic cells. Values represent mean ± SD from three independent experiments. **P < 0.01. c Western blot analysis of PCNA, cyclin D1 and cleaved caspase-3 in Si-653 or Si-NC transfected and IHH4 cells. β-actin was used as a loading control