Fig. 5.
Reduction of Nibrin expression induced by chronic hypoxia. (a) Expression plots of NBN mRNA in D283-MED cells incubated in 1% O2 for 0–96 h. Filled circle and dark blue line show the average expression of the transcript. Open circle represents the data point for each individual probe. Numbers above data points show the fold change normalised to basal ratio. (b) NBN mRNA measured by qPCR normalised to cyclophillin A and the normoxic (21% O2) control. (c) NBN protein levels over time in hypoxia (0–96 h), measured by western-blot. (d) Levels of NBN, MRE11 and RAD50 mRNA measured by qPCR, normalised as in (b). (e) Levels of NBN, MRE11 protein after 5 days hypoxic (1% O2) or normoxic (21% O2) exposure, measured by western-blot. Data shown are ± S.E.M of at least 3 independent experiments. Densitometry quantification for western blots using Image J. * denotes significance (p < 0.05) as determined by student t-test