Fig. 7.

Etoposide efficacy is not affected by hypoxia. D283-MED cells were incubated in 1% O₂ for 5 days or in 21% O₂ prior to etoposide (20 μM) treatment for indicated time points. (a) ɣH2AX bound secondary Cy3 antibodies were detected by immunofluorescence, staining intensity was quantified for individual cells using AQM analysis. Data shown are the mean ± S.E.M of three independent experiments (n > 350 cells). One-way ANOVA followed by Bonferroni test was performed (*indicates p < 0.05). Images are of a single representative experiment. Quantitative data are represented as a fold change relative to T0. (b) Comet assay (performed according to manufacturer’s protocol). Percentage of ‘Olive Tail moment’ was calculated (see methods section). Results shown are the mean ± S.E.M of 3 independent experiments (n > 100cells). One-Way ANOVA followed by Bonferroni test performed (*indicated< 0.05)