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. 2018 Apr 13;39(4):740–750. doi: 10.1177/0271678X18768955

Figure 2.

Figure 2.

Caffeine does not affect frequency of KCl-induced cortical spreading depolarization in mice.(a) CSD frequency was assessed by topical cortical application of KCl and represented as the number of CSDs per hour. (b) Representative electrophysiological tracings of CSD frequency measurement for each treatment group. (c) Whisker box plots show that acute (0 mg/kg n = 9; 30 mg/kg n = 9; 60 mg/kg n = 9; 120 mg/kg n = 10) or chronic (0 mg/kg/day n = 10 (1 week); 60 mg/kg/day n = 5 (1); 240 mg/kg/day n = 7 (1 week); 60 mg/kg/day n = 5 (2 weeks)) caffeine administration did not affect CSD frequency. The results were not affected by the choice of anesthetic (isoflurane vs. urethane (n = 7 per group)). Similarly, caffeine withdrawal (0 mg/kg/day n = 10; 120 mg/kg/day n = 8 (1 week); 240 mg/kg/day n = 8 (1 week)) had no effect on CSD frequency in C57BL/6J mice (p > 0.05). The ends of the whiskers represent minimal and maximal data points. The horizontal lines within the box indicate the median, and the “+” sign represents the mean. Open circles show individual data points.