Table 1.

Systemic physiologic parameters, and characteristics of cortical spreading depolarization.

Group				Systemic physiology						CSD
Treatment	Genotype	Caffeine	Number of Animals	Age (mo)	BW (g)	BP	pH	pCO2	pO2	Speed (mm/min)	Duration (s)	Amplitude (mV)
Acute	C57BL/6 J	Vehicle	9	4 ± 1	22 ± 3	79 ± 9	7.37 ± 0.04	31 ± 4	135 ± 21	3.3 ± 0.9	29 ± 6	17 ± 3
(isoflurane)		30 mg/kg	9	4 ± 1	23 ± 3	82 ± 9	7.34 ± 0.03	30 ± 5	134 ± 22	3.5 ± 0.9	32 ± 9	17 ± 4
		60 mg/kg	9	3 ± 1	22 ± 2	79 ± 7	7.35 ± 0.04	32 ± 5	144 ± 18	3.7 ± 0.5	33 ± 10	19 ± 2
		120 mg/kg	10	5 ± 1	24 ± 3	79 ± 6	7.35 ± 0.05	26 ± 3	143 ± 17	3.9 ± 0.8	27 ± 7	20 ± 3
Acute		Vehicle	7	2	18 ± 1	78 ± 7	7.37 ± 0.05	38 ± 7	120 ± 18	3.9 ± 0.5	33 ± 8	19 ± 2
(urethane)		120 mg/kg	7	2	18 ± 1	77 ± 8	7.36 ± 0.06	39 ± 7	124 ± 19	3.8 ± 0.8	29 ± 6	19 ± 2
Chronic	C57BL/6 J	Vehicle (one week)	10	5 ± 1	22 ± 2	85 ± 11	7.37 ± 0.04	28 ± 4	149 ± 13	4.2 ± 0.5	33 ± 13	19 ± 2
		60 mg/kg/d (one week)	5	2	18 ± 1	81 ± 12	7.39 ± 0.07	38 ± 8	119 ± 12	4.2 ± 0.5	33 ± 13	19 ± 2
		240 mg/kg/d (one week)	7	5	18 ± 2	76 ± 4	7.33 ± 0.03	27 ± 4	140 ± 12	4.2 ± 1.0	23 ± 6	18 ± 2
		Vehicle (two weeks)	5	2	18 ± 2	77 ± 11	7.37 ± 0.06	39 ± 8	149 ± 13	3.6 ± 0.3	38 ± 17	19 ± 4
		60 mg/kg/d (two weeks)	5	2	18 ± 1	76 ± 9	7.39 ± 0.07	37 ± 7	140 ± 12	3.4 ± 0.4	39 ± 12	22 ± 2
Withdrawal	C57BL/6 J	Vehicle	10	5 ± 1	22 ± 2	85 ± 11	7.37 ± 0.04	28 ± 4	149 ± 13	4.2 ± 0.5	33 ± 13	22 ± 4
		120 mg/kg/d	8	5 ± 1	22 ± 2	86 ± 8	7.39 ± 0.04	28 ± 4	153 ± 8	3.8 ± 0.8	31 ± 11	22 ± 2
		240 mg/kg/d	8	4 ± 1	25 ± 3	88 ± 11	7.40 ± 0.05	27 ± 3	146 ± 16	4.4 ± 0.6	34 ± 8	22 ± 3
	R192Q	Vehicle	6	5 ± 2	21 ± 2	83 ± 5	7.37 ± 0.03	29 ± 3	135 ± 8	5.8 ± 1.0	29 ± 6	25 ± 3
		240 mg/kg/d	7	5 ± 2	21 ± 2	89 ± 11	7.38 ± 0.03	30 ± 3	143 ± 19	6.5 ± 1.8	31 ± 8	24 ± 5

CSD: cortical spreading depolarization.

Note: Data are mean ± SD. Systemic physiologic parameters and characteristics of CSD did not differ between caffeine treatment groups (p > 0.05).