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. 2019 Apr 3;15:104. doi: 10.1186/s12917-019-1839-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Scheme of sex chromosome-specific probe production. DNA sequences of both type of probes are initially amplified from bull genomic DNA and secondly from the products of the first PCR. After amplification the probes are labelled with mono-reactive dyes and vacuum dried until use