Table 1.

In silico analysis of predicted BALB/c MHC class I epitopes.

Antigenicity Score	Percentile Rank	Allele	Length	*Position	Peptide
Predicted Model 1
0.400	0.4	H-2-Dd	9	15-23	ISPRTLNAW
0.658	0.4	H-2-Dd	9	32-40	FSPEVIPMF
1.763	0.4	H-2-Dd	9	363-371	PGPGVRYPL
-0.099	0.5	H-2-Kd	9	142-150	VRMYSPTSI
0.450	0.6	H-2-Kd	9	233-241	AYMGGKWSK
0.228	0.7	H-2-Kd	9	7-15	QGQMVHQAI
0.324	0.7	H-2-Dd	9	83-91	VHPVHAGPI
1.880	0.7	H-2-Dd	9	222-230	GGPGHKARV
0.786	1	H-2-Ld	9	2-10	IVQNIQGQM
-0.317	1	H-2-Dd	9	88-96	AGPIAPGQM
Antigenicity Score	Percentile Rank	Allele	Length	*Position	Peptide
Predicted Model 5
-0.099	0.5	H-2-Kd	9	63-71	VRMYSPTSI
0.324	0.7	H-2-Dd	9	4-12	VHPVHAGPI
1.880	0.7	H-2-Dd	9	143-151	GGPGHKARV
-0.317	1	H-2-Dd	9	9-17	AGPIAPGQM
1.763	0.4	H-2-Dd	9	165-173	PGPGVRYPL
Predicted Model 6
1.763	0.4	H-2-Dd	9	165-173	PGPGVRYPL
-0.099	0.5	H-2-Dd	9	63-71	VRMYSPTSI
0.324	0.7	H-2-Dd	9	4-12	VHPVHAGPI
1.880	0.7	H-2-Dd	9	143-151	GGPGHKARV
-0.317	1	H-2-Dd	9	9-17	AGPIAPGQM

BALB/c MHC I interacting with 9-mer peptides of predicted models were sorted based on percentile rank calculated by IEDB tools. Model 1: Full p24-AAY-Nef, Model 5: p24 (80-231)-AAY-Nef (70-90), Model 6: p24 (80-231)-AAY-Nef (120-150).

*Positions for peptides are as: Model 1:1-231 aa of p24, 232-234 aa of linker, 234-440 aa of Nef.

Model 5: 1-152 aa of p24, 153-155 aa of linker, 156-176 aa of Nef.

Model 6: 1-152 aa of p24, 153-155 aa of linker, 156-186 aa of Nef

Antigenicity scores ≥ 0.5 are shown in bold.