Figure 3.

a) Encapsulation (Encap.) efficiency (= weight %) of PEI‐5, PEI‐25 by in situ loading and postloading; determined by a combination of shear force‐driven HFF purification and UV–vis spectroscopy. b) Release kinetics of PEI from polymersomes at pH 5 and 8 given as percentage of encapsulated PEI. c) Loading efficiency of esterase and myoglobin by in situ loading and postloading determined by AF4—using unpurified samples. Further details on loaded enzyme per polymersome as percentage are given in Table S8 (Supporting Information). For example, Myoglobin in situ ranges from ≈15 and ≈41%, when adding different amounts of enzymes to polmersome without purifying the samples for AF4 study. Note that results of Figure 3a,c are not comparable due to differently applied methods with/without purification steps.