Fig. 4.

Melatonin receptor (MT2) participates in lipolysis in precursor adipocytes. A: The mRNA levels of MT1 and MT2 mRNA were measured in melatonin-treated mature adipocytes by RT-qPCR. B, C: Western blot analyses were performed to detect the changes of MT1 and MT2. D–F: RT-qPCR analysis of genes involved in lipolysis in mature adipocytes cultivated in GM, GM + melatonin, and GM + melatonin + 4-P-PDOT medium for 48 h. Data are represented as the mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3).