TABLE 1.
Gene | Primer Sequences | Product Size (bp) | Temperature (°C) |
C/EBPα | 5′ ACACGGTGCGTCTAAGATGAG | 238 | 59.1 |
5′ TCGGAGCGGTGAGTTTGC | |||
FABP4 | 5′ GGTGTCACGGCTACCAGAA | 203 | 60.3 |
5′ AAATCATTACATCGCCTTAT | |||
FASN | 5′ GCCAGCATCGTGAGGGTC | 196 | 58.6 |
5′ CCAGTGCAGGTACGGGAAT | |||
PPARγ | 5′ GCCATTCGCATCTTTCAGGG | 213 | 59.3 |
5′ CGTGGACGCCATACTTTAGGA | |||
HSL | 5′ TGTCTTTGCGGGTATTCGG | 241 | 59.1 |
5′ GCCTGTTTCATTGCGTTTGTA | |||
ATGL | 5′ TGGATAAAGGAGCAGACAGG | 216 | 60 |
5′ TCGGACGGGAAGGCAGT | |||
PLIN1 | 5′ TGAAATGCCACGACAA | 187 | 60.5 |
5′ CTACGGCTAAACTTACTACAC | |||
UCP3 | 5′ TCGGAAATAGGAGGAGGAAA | 270 | 58.8 |
5′ TCGGCTGATTTCCAAGTGT | |||
Tfam | 5′ CCTAGGAAGCGACTACTGCG | 126 | 59.7 |
5′ GCAACTCTTCAGACCTCGCT | |||
CPT-1β | 5′ TGGGGCTGGTCAATCACATC | 188 | 59.1 |
5′ GCCGTGCATCTCAAACATCC | |||
CPT-1α | 5′ GTCCTGCAACTTTGTGCTGG | 97 | 60 |
5′ CAGGTGCTGGTGCTTTTCAC | |||
Pgc1a | 5′ GGACTGACATCGAGTGTGCT | 246 | 60.3 |
5′ GCGTCTCTGTGAGAACTGCT | |||
Cidea | 5′ GGCTCACAAGTGGATAGGGG | 250 | 60.1 |
5′ AGATGAAGAGGAAGCATGGAAGT | |||
Prdm16 | 5′ CCACAAGTCCTACACGCAGT | 160 | 60.3 |
5′ CGGGTAATGGTTCTTGCCCT | |||
Cox2 | 5′ CATACTAACAACAAAACTGACA | 346 | 59.3 |
5′ TGTCAGTTTTGTTGTTAGTATG | |||
β-globin | 5′ CAACCTCAAGGGCACCTTTGCTA | 150 | 60 |
5′ GGCCCTCTCTTCATGACACG | |||
RPLP0 | 5′ GTGCTGATGGGCAAGAAC | 225 | 59.6 |
5′ CTCCAGGAAGCGAGAATG |