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. 2018 Dec 14;60(4):767–782. doi: 10.1194/jlr.M087619

TABLE 1.

Primers used in this study

Gene Primer Sequences Product Size (bp) Temperature (°C)
C/EBPα 5′ ACACGGTGCGTCTAAGATGAG 238 59.1
5′ TCGGAGCGGTGAGTTTGC
FABP4 5′ GGTGTCACGGCTACCAGAA 203 60.3
5′ AAATCATTACATCGCCTTAT
FASN 5′ GCCAGCATCGTGAGGGTC 196 58.6
5′ CCAGTGCAGGTACGGGAAT
PPARγ 5′ GCCATTCGCATCTTTCAGGG 213 59.3
5′ CGTGGACGCCATACTTTAGGA
HSL 5′ TGTCTTTGCGGGTATTCGG 241 59.1
5′ GCCTGTTTCATTGCGTTTGTA
ATGL 5′ TGGATAAAGGAGCAGACAGG 216 60
5′ TCGGACGGGAAGGCAGT
PLIN1 5′ TGAAATGCCACGACAA 187 60.5
5′ CTACGGCTAAACTTACTACAC
UCP3 5′ TCGGAAATAGGAGGAGGAAA 270 58.8
5′ TCGGCTGATTTCCAAGTGT
Tfam 5′ CCTAGGAAGCGACTACTGCG 126 59.7
5′ GCAACTCTTCAGACCTCGCT
CPT-1β 5′ TGGGGCTGGTCAATCACATC 188 59.1
5′ GCCGTGCATCTCAAACATCC
CPT-1α 5′ GTCCTGCAACTTTGTGCTGG 97 60
5′ CAGGTGCTGGTGCTTTTCAC
Pgc1a 5′ GGACTGACATCGAGTGTGCT 246 60.3
5′ GCGTCTCTGTGAGAACTGCT
Cidea 5′ GGCTCACAAGTGGATAGGGG 250 60.1
5′ AGATGAAGAGGAAGCATGGAAGT
Prdm16 5′ CCACAAGTCCTACACGCAGT 160 60.3
5′ CGGGTAATGGTTCTTGCCCT
Cox2 5′ CATACTAACAACAAAACTGACA 346 59.3
5′ TGTCAGTTTTGTTGTTAGTATG
β-globin 5′ CAACCTCAAGGGCACCTTTGCTA 150 60
5′ GGCCCTCTCTTCATGACACG
RPLP0 5′ GTGCTGATGGGCAAGAAC 225 59.6
5′ CTCCAGGAAGCGAGAATG