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. 2019 Jan 27;60(4):783–793. doi: 10.1194/jlr.M088807

Fig. 2.

Fig. 2.

Coimmunoprecipitation of ANGPTL complexes. Western blot of fractions obtained from coimmunoprecipitation of refolded ANGPTL complexes by protein G beads coated with an anti-ANGPTL8 Ab for ANGPTL3/ANGPTL8 complexes or with anti-ANGPT4 Ab for ANGPTL4/ANGPTL8 complexes. ANGPTL8T stands for the truncated ANGPTL8 protein with residues 172–198 removed. Fractions were as follows: E, elution; S, starting material; U, unbound material; W, washes 1–3. A: A control experiment with the individual proteins and anti-ANGPTL8 protein G beads. B: Complexes between ANGPTL3 and full-length ANGPTL8 captured on anti-ANGPTL8 protein G beads. C: Complexes between ANGPTL3 and truncated ANGPTL8 captured on anti-ANGPTL8 protein G beads. D: Control experiment with the individual proteins and anti-ANGPTL4 protein G beads. E: Complexes between ANGPTL4 and full-length ANGPTL8 on anti-ANGPTL4 protein G beads. F: Complexes between ANGPTL4 and truncated ANGPTL8 on anti-ANGPTL4 protein G beads.