Fig. 5.

LD remodeling was blocked by both orlistat and triacsin C while mitochondrial rearrangement was blocked by triacsin C only. After culturing Om adipose tissues with or without Rosi for 5 days, triacsin C or orlistat was added during the final 2 days of culture without or with Rosi, as described in the Materials and Methods. Adipocytes were isolated and stained with LipidTOX-Deep Red and Mitotracker-Red followed by confocal imaging. White scale bars = 10 μm.