Fig. 7.

Rosi increased oxidative capacity in newly differentiated subcutaneous adipocytes. A: Differentiated abdominal subcutaneous adipocytes (day 14) were treated with Rosi for 7 days and protein levels of UCP1, PLIN5, FABP3, and porin were measured. Blots from three different individuals are shown. B: OCRs were measured with a Seahorse extracellular flux analyzer. After measuring basal OCR, the following were injected sequentially: oligomycin (ATP synthase inhibitor), FCCP (uncoupler), and rotenone (complex I inhibitor)/antimycin A (complex III inhibitor) (Rot/AA). The mean ± SD of five wells per condition over time from a representative subject is shown. C: Average values for different components of mitochondrial respiration were calculated (43). Data are expressed as the mean ± SEM of values from three independent subjects (**P < 0.01, ***P < 0.001 by paired t-tests for Rosi effects).