Fig. 3.

SHP does not contribute to CYP7A1 repression by atRA. HepaRG cells were transfected with siRNA (50 nM) targeting SHP (siSHP) or control siRNA (siCon), followed by treatment with atRA (1 µM) or DMSO for the time periods indicated. mRNA and protein levels were determined by qPCR (n = 3, mean ± SD) (A, D) and Western blot analysis (B, C), respectively.