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. 2018 Dec 13;97(2):229–235. doi: 10.1111/imcb.12216

Figure 4.

Figure 4

AMD3100 effect on acute myeloid leukemias (AML) and T‐cell acute lymphoblastic leukemia (T‐ALL) cell clusters. Representative maximum projections from intravital microscopy (IVM) three‐dimensional stacks acquired prior to and 180 min following AMD3100 administration, showing the effect of the drug on AML (a) and T‐ALL (c) cell clusters. CXCR4 antagonism produced (b) minimal effect on AML cluster sizes and (d) a significant reduction in early colonizing and chemoresistant T‐ALL cell clusters as quantified by paired analysis of mean fluorescence intensity of the same areas before and 180 min after AMD3100 injection. (a, b) Data obtained from four mice with early AML infiltration and three mice with chemoresistant AML (paired t‐test). Data are from two independent experiments. (c, d) Data obtained from three mice with early T‐ALL infiltration and four mice with chemoresistant T‐ALL. Data are from two independent experiments. Red: AML/T‐ALL cells; green: Flk1‐GFP + endothelial cells; blue: Cy5‐labeled blood vessels. *P < 0.05, **P < 0.01. Paired t‐test.