Skip to main content
. 2019 Feb 1;179(4):1834–1843. doi: 10.1104/pp.18.01261

Figure 6.

Figure 6.

Complementation of 17ab with various mutant forms of IRE1b. IRE1b, IRE1bD608N, K610N, IRE1bD628A (the latter two are protein kinase dead), and IRE1bN820A (RNase dead) were introduced into the 17ab mutant background by agrobacteria-mediated transformation. A, Reverse transcription (RT)-PCR analysis of bZIP60(s) (spliced form of bZIP60 mRNA) and IRE1b in the wild type (WT), 17ab, and different transgenic plants. ACTIN2 was used as a loading control. B, The wild type, 17ab, and different transgenic plants were grown under short-day conditions (8 h of light and 16 h of dark) for 45 d. Rosette images were digitally abstracted for comparison. C, Seedlings of different genotypes listed above were grown vertically on one-half-strength MS medium with a 16-h-light/8-h-dark cycle for 7 d. D, Root lengths of the different genotypes shown in B and C. Data represent means ± sd of three independent experiments. One-way ANOVA with Tukey’s method was used to examine significant differences at P < 0.01 in pairwise comparison and classified as a or b.