Ler and Sha have similar NCED3 expression, while the Sha and Sha-like NCED3 haplotypes are associated with lower ABA accumulation. A, NCED3 gene expression in Ler and Sha in the unstressed control (time 0) and after transfer to –1.2 MPa (10 and 96 h). Data are means ± se (n = 3). A significant difference (Student’s t test, P ≤ 0.05) of Ler versus Sha at the 0-h time point is indicated by the asterisk. The results are representative of several independent experiments. B, ABA accumulation of accessions with the Sha and Sha-like NCED3 haplotypes compared with 298 accessions phenotyped for ABA accumulation (Kalladan et al., 2017). The Sha haplotype group had significantly different ABA accumulation than the 298 accessions (Student’s t test, P ≤ 0.05). Whiskers show the 10th and 90th percentiles, and dots indicate outliers. Accessions with the Sha and Sha-like NCED3 haplotypes are listed in Supplemental Table S1. FW, Fresh weight.