Figure 4.

Delivery of CSCs to the PM is reduced in myosin-deficient cells. A, Representative single-frame images of PM-localized CSC particle recovery after photobleaching. Seedlings of wild type (WT) or xi3KO were mounted in mock (0.2% DMSO), 30 mm BDM, 10 µm PBP, or 10 µm LatB just prior to imaging. A region of interest at the PM was photobleached and the number of newly delivered CSCs was counted in a subarea within the region (yellow dashed box). Bar, 5 µm. B, The rate of delivery of CSCs to the PM was calculated from the total number of newly delivered CSCs during the initial 5 min of recovery divided by the measured area and time. The CSC delivery was significantly inhibited in xi3KO and BDM-, PBP-, and LatB-treated cells compared with mock-treated wild-type sibling. Values given are means ± se (n ≥ 11 cells per genotype or treatment, one-way ANOVA with Tukey’s post-hoc test, letters [a–c] denote samples/groups that show statistically significant differences with other groups, P < 0.05).