Figure 1.
The patterns of up-regulated genes in crwn mutants demonstrate both synergistic and antagonistic relationships among crwn mutations. The sizes of each circle in the Venn diagrams are proportional to the number of genes up-regulated at least 2-fold in our RNA-seq data (q < 0.01). A, Up-regulated genes in three crwn single mutants, crwn1, crwn2, and crwn4, showing shared transcriptomic alterations. B, Up-regulated genes in the double mutant crwn1 crwn2 and corresponding single mutants, crwn1 and crwn2, showing the synergistic effects of the crwn1 and crwn2 mutations. C, Up-regulated genes in the double mutant crwn1 crwn4 and corresponding single mutants, crwn1 and crwn4, showing the antagonistic effects of these mutations. D, Up-regulated genes in two double mutants, crwn1 crwn2 and crwn1 crwn4, and one single mutant, crwn4, illustrating overlapping profiles. Note that PR1 and PR2 fell in the overlap between crwn1 crwn2 and crwn1 crwn4 mutants (this overlap contains 119 genes), and PR5 was found only in the sector corresponding to the crwn1 crwn2 mutant (region with 565 genes).