PP2-A5 (AT1G65390) gene expression in Arabidopsis Bla-2, Kon, and Columbia-0 (Col-0) accessions in response to T. urticae infestation and in different Col-0 tissues. A, Microarray data from the PP2-A5 expression in Arabidopsis Bla-2 and Kon accessions at 1, 3, 6, 12, and 24 h postinfestation represented as log2. B, Microarray validation by RT-qPCR assays of PP2-A5 gene expression at 1, 3, 6, 12, and 24 h postinfestation. Gene expression is referred to as relative expression levels (2^-dCt). C, Relative gene expression levels (2^-dCt) of PP2-A5 in different Arabidopsis Col-0 tissues: flowers (F), roots (R), seeds (S), siliques (Si), leaves from stem (Ls), and leaves from rosettes at 1, 2, and 3 weeks old (LR1, LR2, and LR3). For B and C, data are means ± se of three biological replicates. Different letters indicate significant differences between times postinfestation within each accession or among different tissues (P < 0.05, one-way ANOVA followed by Tukey’s multiple comparisons test).