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. 2019 Jan 29;179(4):1416–1430. doi: 10.1104/pp.18.01594

Figure 9.

Figure 9.

Competition between OsMBL1 and MoChi1 in the binding of chitin. A, Purified recombinant OsMBL1 (10−5 μmol) was mixed with various amounts of MoChi1 (from 0, 10−5 to 10−2 μmol) prior to the addition of chitin beads (100 μL). B, Recombinant SSB-MoChi1-6*His proteins (10−4 μmol) were incubated with OsMBL1 in the presence of increasing amounts (from 0, 10−5 to 10−2 μmol) prior to the addition of chitin beads (100 μL for each). After further incubation, insoluble chitin beads were rinsed and collected for SDS-PAGE followed by immunoblots. Recombinant GST-OsMBL1 proteins (43 kDa) and SSB-MoChi1-6*His (73kDa) proteins were detected by anti-GST and anti-His antibodies, respectively. C, Influence of MoChi1 and OsMBL1 on chitin-triggered ROS burst in rice suspension cells. Production of ROS after induction with 8 nm (GlcNAc)6 was measured in the absence or presence of MoChi1 or OsMBL1. The experiment was performed three times with similar results. The values represent means ± se of three independent tests.