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. 2019 Feb 26;218(4):1422. doi: 10.1083/jcb.20090303002222019c

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© 2019 Francavilla et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 6. — Src inhibition blocks the recycling of FGFR1 and promotes the association of Cbl with FRS-2α in NCAM-stimulated cells. (A) HeLa cells stimulated with FGF-2 or FGL in the presence of DMSO (top), PP1 (middle), or SU6656 (bottom) were processed as for Fig. 3 A. Asterisk marks a cell where HA-FGFR1 recycled back to the cell surface. Bar, 10 µm. (B) HeLa cells were treated with DMSO (left), PP1 (middle), or SU6656 (right) before stimulation with either FGF-2 or FGL for 10 min. Cell extracts were immunoprecipitated (IP) with anti–FRS-2α antibody and immunoblotted for Cbl (top) followed by immunoblotting for FRS-2α (bottom).