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. 2019 Feb 20;218(4):1118–1127. doi: 10.1083/jcb.201809027

Figure 3.

Figure 3.

Overriding RIDD of Blos1 sensitizes cells to ER stress. (A–D and G) We treated MC3T3-E1 cells stably expressing the indicated mRNAs with Tg or Tm (18 h), 2 mM DTT, arsenite (6 h), or serum-free media (18 h). We then counted live cells or washed out the DTT and counted cells after 24 h. (E and F) We treated cells with DTT (2 mM, 4 h) or arsenite (50 µM, 6 h) and measured Blos1 mRNA by qPCR and LE/lysosome clustering by LAMP1 immunostaining. (H and I) We incubated cells with Tg (4 µM) or serum-free media for 18 h, then measured cleaved caspase 3 levels by immunoblotting. For A, B, and D: P values were determined by two-factor ANOVA and compared Rfp with Blos1s cell lines. For C and I: *P < 0.05, paired t test for Rfp versus Blos1s cell lines, corrected for multiple comparisons. n = 3 except where indicated. UT, untreated; OE, overexpressed; Ars, arsenite; SS, serum starvation.