Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 6;218(4):1250–1264. doi: 10.1083/jcb.201805036

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Adriaans et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — PLK1-independent furrow ingression requires centralspindlin. (A) Western blot of samples derived from HeLa cells transfected with either siLuc or siRACGAP1. The Western blot was probed with an anti-RACGAP1 antibody. α-Tubulin is shown as loading control. (B) HeLa cells were transfected with siLuc or siMKLP1 and processed for IF (right). The percentage of cells with detectable MKLP1 in anaphase was scored and used as a measure for knock-down efficiency (left). The number of cells that were scored per condition is indicated (n). One representative experiment out of two is shown. ****, P < 0.0001; χ² test for comparison of the indicated conditions. (C and D) HeLa cells transfected with the indicated siRNAs were imaged live. PLK1 was inhibited by addition of BI2536 (100 nM) before anaphase onset (PLK1 inh.). PLK1 was inhibited by addition of BI2536 (100 nM) before anaphase onset. The number of cells showing complete furrow ingression, full-furrow ingression followed by furrow regression, visible but minimal furrow ingression, or no furrow ingression was scored. The number of cells analyzed per condition is indicated (n). One representative experiment out of two is shown. ****, P < 0.0001; ***, P < 0.001; χ² test for comparison of the indicated conditions; ns, not significant. (E) DIC stills of HeLa cells transfected with the indicated siRNAs with or without addition of BI2536 (100 nM) before anaphase onset. Bars: 10 µm. Stills of more time frames are shown in Fig. S1 D.