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. 2019 Feb 6;218(4):1250–1264. doi: 10.1083/jcb.201805036

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© 2019 Adriaans et al.

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Figure 4. — Aurora B activity at the equatorial cortex is required for spindle midzone–independent furrow ingression. (A) Representative brightfield stills of a live cell imaging experiment with HeLa cells transfected with the indicated siRNAs and treated with (AurB inh.) or without the Aurora B inhibitor ZM447439 (2 µM) before anaphase onset. (B) Cells were treated and imaged as in A, and the percentage of cells showing either stable furrow ingression, furrow ingression followed by furrow regression, minimal furrow ingression, or no furrow ingression was scored. The number of cells analyzed per condition is indicated (n). One representative experiment out of two is shown. ****, P < 0.0001; χ² test for comparison of the indicated conditions; ns, not significant. (C) IF for Anillin and RhoA. DNA was visualized using DAPI. (D) Quantification of the fluorescence intensity levels of RhoA at the equatorial cortex in anaphase. Each dot represents an individual cell. Error bars depict the SD of the mean. ****, P < 0.0001; Student’s t test for comparison of the indicated conditions. Bars: 10 µm (A and C).