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. 2019 Mar 15;216(4):831–846. doi: 10.1084/jem.20181604

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© 2019 Kimura et al.

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Figure 6. — The loss of Sox8 causes delay in the production of symbiotic bacteria-specific IgA antibody after weaning. (A) Follicle size of PPs measured by stereomicroscope; *, P < 0.05; Student’s t test; n = 7 from Sox8^+/+ mice, n = 5 from Sox8^−/− mice. (B) The gating strategy to analyze the B and T cell populations in ileal PPs. B (CD3ε⁻B220⁺) cells were analyzed for IgA⁺ B and CD95⁺GL7⁺ GC B cells. T (CD3ε⁺B220⁻) cells were analyzed for total CD4⁺ Th, CD8α⁺ cytotoxic T (Tc), and CD4⁺CD8α⁻CXCR5⁺PD-1⁺ Tfh cells. (C and D) Flow cytometry analysis of indicated immune cells in ileal PPs. (C) Number of total B cells, GC B cells, and IgA⁺ B cells. (D) Number of total T cells, Tc cells, Th cells, and Tfh cells; *, P < 0.05; **, P < 0.01; n.s., not significant; Student’s t test, n = 3 per group. (E) Fecal IgA concentration measured by ELISA; *, P < 0.05; Student’s t test; n = 5 from Sox8^+/+ mice, n = 4 from Sox8^−/− mice (4 and 6 wk old); n = 5 per group (8 wk old). (F) Flow cytometry analysis of IgA-coated bacteria in feces; **, P < 0.01; Student’s t test; n = 5 from Sox8^+/+ mice, n = 4 from Sox8^−/− mice. (G) Mice were orally infected with 5 × 10⁷ CFU of rSalmonella-ToxC. The presence of TT-specific IgA in feces and IgG in serum at 2 and 3 wk after immunization were evaluated by ELISA; Student’s t test; n = 10 from Sox8^+/+ mice, n = 6 from Sox8^−/− mice. Data are representative (B–F) or means (A and G) of two independent experiments. wpi, weeks post-immunization. All values are presented as the mean ± SD.