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CTFR labeling is improved by pre‐sorting narrow peak of undivided cells. Murine splenic naïve B cells labeled with 7.5 μmol L−1 CTFR were cultured in anti‐CD40 and IL‐4 for 10 h, then (a) left unsorted (orange) or (b) sorted into a narrow undivided peak (red) before being plated into triplicate wells and analyzed at specified times after initial culture. Data are representative of 3 independent experiments. CTFR, CellTrace Far Red.
