Figure 5.

CTY increases the number of cell divisions that can be defined. Human naïve and memory B cells were labeled with CTV (5 μmol L⁻¹) or CTY (20 μmol L⁻¹) and cultured in the presence or absence of CD40L and IL‐21 for 7 days, and re‐stimulated after 4 days. Division profiles with the number of cell divisions is shown for (a) CTV‐labeled (purple) and (b) CTY‐labeled (yellow) naïve (left) and memory (right) B cells after 7 days of culture. For each plot, unlabeled, activated cells (gray) represent autofluorescence for each cell tracking dye, and labeled, unstimulated cells (blue) indicate the fluorescence level of undivided cells. Data are representative of 2 independent experiments. CTY, CellTrace Yellow; CTV, CellTrace Violet.