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. Author manuscript; available in PMC: 2019 Apr 3.
Published in final edited form as: Exp Physiol. 2018 Sep 13;103(11):1469–1480. doi: 10.1113/EP087207

6. Effects of chronic rapamycin on mTORC signaling in subcutaneous adipose tissue of obese mice.

6.

(A-B) Phosphorylation status of p70S6K1, S6, Akt and total rictor (component of mTORC2) were immunoassayed in tissue lysates prepared from mice consuming microencapsulated rapamycin (Rapa) or placebo (P) containing HFHS diets. Animals were fasted overnight (~16h) and injected with insulin (1U/kg) prior to sacrifice. Inguinal white adipose tissue (IWAT) was harvested 10 minutes after insulin injection. Saline was injected as control in some mice (as depicted in the figures). Tubulin was probed to assess for adequacy of protein loading. White bars: placebo-fed mice; black bars: rapamycin-fed mice, *p<0.05, ***p<0.001 vs placebo.