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Nutrient profile of phosphorus, acetate, nitrogenous compounds, and oxygen concentration in the laboratory-scale SBR on day 522. Dotted lines separate the anaerobic (ANA), microaerobic (AER), and settling (SET) periods. Red bars indicate the time points used for RNA-seq (letters in red correspond to sample names). The periods of addition of media A (containing acetate and phosphate) and media B (containing ammonia) are indicated for the anaerobic stage.
