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. 2019 Jan 15;4(1):e00329-18. doi: 10.1128/mSystems.00329-18

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Copyright © 2019 Hazen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — ST production by selected CFA/I- or CS6-producing ST-only ETEC isolates. ST production was measured by ST-mediated accumulation of cGMP (in picomoles per milliliter) in T84 cell monolayers cultured on 24-well tissue culture-treated plates. T84 monolayers were pretreated with the phosphodiesterase inhibitors zardaverine and vardenafil followed by the addition of 10 μl of ETEC cell culture supernatant from shaking cultures grown in 4AA medium or known masses of ST (BEI Resources; NR-50765). The values represent means and standard deviations of results from triplicate assays. The amount of cGMP in the supernatant of each of the ETEC isolates was calibrated to the amount of cGMP produced by treatment of T84 monolayers with 10 ng of purified STh to determine the amount of ST (in nanograms per microliter) produced by the ETEC isolates. The CFA/I ETEC isolates are indicated with blue isolate labels, while the CS6 ETEC isolates are indicated with green isolate labels (see inset legend). The bars are colored according to the phylogenomic lineage.