TABLE 2.
Summary of ETEC virulence factor content in the CS6- and CFA/I ST-only ETEC genomes
| Virulence factor | GenBank accession no. or sourcea | No. (%) of genomesb
|
P valuec | ||
|---|---|---|---|---|---|
| All CFA/I and CS6 |
CFA/I | CS6 | |||
| Toxins | |||||
| STIa (STp) | YP_003294006.1 | 1 (<1) | 0 (0) | 1 (1) | NS |
| STIb (STh) | WP_023485648.1 | 242 (90) | 147 (91) | 95 (89) | NS |
| LT-I | YP_003293996.1–YP_003293997.1 | 4 (1) | 2 (1) | 2 (2) | NS |
| EAST1 | AAD43571.1 | 80 (30) | 37 (23) | 43 (40) | 0.0036 |
| Colonization factors and adhesins | |||||
| CFA/I | CBJ04486.1–CBJ04489.1 | 144 (54) | 143 (88) | 1 (1) | <0.001 |
| CS6 | AAC45093.1–AAC45096.1 | 89 (33) | 0 (0) | 89 (83) | <0.001 |
| CS2 | CAA87760.1–CAA87763.1 | 1 (<1) | 1 (<1) | 0 (0) | NS |
| CS3 | CAA34815.1–CAA34820.1 | 1 (<1) | 1 (<1) | 0 (0) | NS |
| CS4 | AAK97134.1–AAK97137.1 | 5 (2) | 0 (0) | 5 (5) | 0.0094 |
| CS5 | CAA11821.1–CAA11825.1 | 57 (21) | 0 (0) | 57 (53) | <0.001 |
| CS14 | AAQ20104.1–AAQ20108.1 | 5 (2) | 3 (2) | 2 (2) | NS |
| CS21 | ABU50035.1–ABU50050.1 | 167 (62) | 142 (88) | 31 (29) | <0.001 |
| CS22 | AAD30557.1 | 1 (<1) | 1 (<1) | 0 (0) | NS |
| EtpA (EtpBAC) | AAX13508.1–AAX13510.1 | 152 (57) | 144 (89) | 8 (7) | <0.001 |
| EatA | AAO17297.1 | 203 (75) | 137 (85) | 66 (62) | <0.001 |
| SepA-like | This study | 30 (11) | 2 (1) | 28 (26) | <0.001 |
| SepA | AAL72309.1 | 4 (1) | 2 (1) | 2 (2) | NS |
| Tia | AAB06592.1 | 15 (6) | 13 (8) | 2 (2) | NS |
| TibA | CBJ01643.1 | 38 (14) | 34 (21) | 4 (4) | <0.001 |
| Other | |||||
| T2SSα | CBJ03075.1–CBJ03088.1 | 156 (58) | 131 (81) | 25 (23) | <0.001 |
| T2SSβ | CBJ02728.1–CBJ02741.1 | 246 (91) | 159 (98) | 87 (81) | <0.001 |
a
The GenBank accession numbers are indicated for the protein sequences.
b
Data represent numbers of genomes that had each virulence factor with a BSR of ≥0.8, identified using both TBLASTN and BLASTN. The total numbers of genomes analyzed were as follows: all CFA/I and CS6, n = 269; CFA/I, n = 162; CS6, n = 107. The percentages of the genomes are indicated in parentheses.
c
P values were generated by comparing the number of CFA/I genomes to the number of CS6 genomes that had each virulence factor by the chi square test (or Fisher's exact test when present in ≤5 genomes) using R v.3.4.1. NS, not significant (P value of >0.05).