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. 2019 Mar 26;10:176. doi: 10.3389/fendo.2019.00176

Figure 2.

Figure 2

Palmitic acid reduces insulin sensitivity in N43/5 cells. (A) Representative western blot showing relative levels of AKT phosphorylation (Ser473) induced by 15 min of insulin or PBS treatment in N43/5 cells pre-incubated with PA (100 μM) or BSA for 6 h, with its respective quantification (B). (B) Data are presented as mean ± SEM. Two ways ANOVA followed by Sidak's multiple comparisons test. **p < 0.01, ***p < 0.001. n = 3. (C) Representative images of 2-NBDG uptake in N43/5 cells incubated with vehicle (BSA) or PA for 6 h and then stimulated with insulin 1 nM for 30 min, with its representative quantification (D). Size bar: 10 μm. (D) Data are presented as mean ± SEM. One-way ANOVA followed by Holm-Sidak's multiple comparisons test. **p < 0.01. n = 3.