Figure 7.

SME1 Ensures the Accurate Splicing of Negative Regulators of the Arabidopsis Cold-Acclimation Process.

Validation of IR events identified in negative regulators of the cold acclimation process in sme1-1 plants exposed to low temperature. For each event, the name of the gene containing the retained intron is indicated. A diagram of the pre-mRNA regions, including the considered introns (gray lines) with their relative positions in the representative gene model and the flanking exons (black bars), together with the captures of the corresponding read coverage tracks obtained from the IGV software, is shown in the left. The quantification of retained introns in wild-type (WT), sme1-1, and sme1-2 plants by qPCR assays is displayed in the right. Data represent the mean of three independent experiments. Asterisks indicate significant differences (*P < 0.01, **P < 0.001, ***P < 0.0001) between sme1 mutants and wild-type plants, as determined by one-way ANOVA (Dunnett’s post-hoc test). Error bars indicate the SD.