Figure 8.
CRR1 Interacts with Various Types of Cotton Chitinases and Confers Fungal Resistance.
(A) Yeast two-hybrid assays to detect interactions between CRR proteins and class IV chitinases. Yeast cells containing the indicated plasmids were grown on SD/-Leu/-Trp DO (DDO) plates and SD/-Leu/-Trp/-Ade/-His DO (QDO) plates (containing 40 mg/L X-α-gal) for 3 d.
(B) Yeast two-hybrid assays to detect interactions between CRR1 and other subclasses of chitinases. AD/BD, negative control; AD-T/BD-53, positive control.
(C) CRR1 confers resistance to B. cinerea. Wild-type (WT) and CRR1-overexpressing (OE-2) Arabidopsis plants were infected with B. cinerea spores and imaged at 3 d postinoculation.
(D) CRR1 confers disease resistance to P. parasitica var nicotianae. Wild-type (SR1) and CRR1-overexpressing (OE-6) tobacco plants were infected with P. parasitica var nicotianae and imaged at 8 d post inoculation.