Figure 2.

Gene Cloning and Genetic Characterization of PP4R3A and PP4R3B.

(A) Gene structures of PP4R3A and PP4R3B (gray block, untranslated region; black blocks, exons; black lines, introns). The arrow indicates the point mutation site in sup-e33 (designated as pp4r3a-1), and the black triangles indicate the T-DNA insertion sites.

(B) Both PP4R3A and PP4R3B possess a pleckstrin homology (PH) domain, a suppressor of MEK-1 (SMK-1) domain, and an Armadillo fold.

(C) Morphological phenotypes of 3-week-old Col, pp4r3a-1 (sup-e33 in Col background), pp4r3a-2, pp4r3b-2, pp4r3a-1 pp4r3b-2, and pp44r3a-2 pp4r3b-2 plants. Scale bars = 1 cm.

(D) Determination of miRNA levels in pp4r3a, pp4r3b and pp4r3a pp4r3b by RNA gel blot analysis of small RNAs. The numbers represent relative abundance of small RNAs.